Neuroprotective effect of novel Rho kinase inhibitor FSD-C10 on a mouse model of Alzheimer disease     

GU Qing-fang  YU Jie-zhong  GUO Min-fang  WEI Wen-yue  XIAO Bao-guo  ZHANG Guang-xian  MA Cun-gen 《园艺学报》2019,35(12):2227-2234

AIM: To explore the neuroprotective effect of novel Rho kinase inhibitor FSD-C10 on Alzheimer disease (AD) model of APP/PS1 double transgenic (Tg) mice. METHODS: Male APP/PS1 Tg mice (n=20) at 8 months of age were randomly divided into 2 groups:model group and FSD-C10 treatment group. The mice were treated with normal saline or FSD-C10 (25 mg·kg^-1·d^-1) by intraperitoneal injection, once daily for 2 months. Age-and sex-matched wild-type (WT) mice without treatment were used as the control. The Morris water maze (MWM) test and SMART 3.0 behavioral record system were applied to examine and analyze the spatial cognitive function of the mice. The protein levels and distribution of Aβ, p-tau and synapse-associated proteins such as synaptophysin, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) and postsynaptic density protein 95 (PSD-95) were determined by immunofluorescence staining. The protein levels of phosphorylated amyloid precursor protein at Thr668[p-APP(Thr668)], beta-site APP-cleaving enzyme 1 (BACE1) and synapse-associated proteins in the brain were analyzed by Western blot. RESULTS: Compared with model group, FSD-C10 treatment significantly improved the cognitive function of the APP/PS1 Tg mice, accompanied by reduced Aβ deposition and p-tau level, increased protein level of p-APP (Thr668) in the central nervous system, decreased expression of BACE1, and increased expression of synapse-associated proteins in the brain of the mice (P<0.05). CONCLUSION: FSD-C10 has neuroprotective potential in the APP/PS1 Tg mice. The mechanism may be related to enhancing the non-amyloidogenic APP cleavage pathway, reducing the production of Aβ oligomers, the deposition of senile plaques and the amount of tau protein, up-regulating synapse-associated proteins, and increasing synaptic plasticity.  相似文献   

农业公司研发投入与核心竞争力关系分析     

孟庆军  古家乐 《湖北农业科学》2020,59(18):160-164

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红螯螯虾转录组中的SSR位点信息分析   总被引：1，自引：0，他引：1  

李喜莲  郭建林  李倩  施伟达  黄振远  顾志敏 《湖北农业科学》2020,59(7):207-211

对红螯螯虾转录组测序数据进行分析,并开发引物通过PCR扩增和毛细管电泳检测,进行了引物多态性和通用性分析。从67 369条Unigenes中共检测到22 727个简单重复序列(SSR)位点,并对包含SSR序列的Unigenes进行功能注释。结果表明,红螯螯虾转录组中微卫星序列的类型较为丰富,其中二核苷酸和三核苷酸重复类型出现频率较多,分别占总SSR出现频率的52.67%和44.25%;四核苷酸、五核苷酸和六核苷酸重复类型出现频率较少,分别占总SSR出现频率的2.62%、0.23%和0.24%。SSR重复类型共有68种,其重复次数的范围为5～84次。筛选出SSR引物5对,对浙江本地及台湾2个群体的60个样品进行遗传多样性分析,每对引物平均产生5.6个多态性片段。本研究结果为深入开发红螯螯虾功能性SSR标记奠定了基础,也为开展红螯螯虾分子标记辅助选育提供支持。  相似文献   

Study on Liver-protective Effect of Chinese Herbal Compound Probiotics on Acute Liver Injury Layers     

YU Jia-min  XIE Quan-xi  ZHANG Jian-mei  GU Wei 《中国畜牧兽医》2016,43(7):1904-1909

This experiment was conducted to investigate the liver-protective effect of Chinese herbal compound probiotics (CHCP) on acute liver injury layers.One hundred and eight 1 day old hens were divided into 4 groups with 3 replicates in each group and 9 layers per replicate in trial 1.The layers in model groups Ⅰ to Ⅲ were gavaged with 10% (V/V) soybean oil solution of carbon tetrachloride (SCCl₄) according to 1,2 and 4 mL/(kg·BW) at 14,28 and 35 d,respectively.The layers in control group were gavaged with 2 mL/(kg·BW) soybean oil.In trial 2,sixty 1 d layers were divided into 5 groups:Control group (soybean oil),model control group(SCCl₄)and low-dose,middle-dose and high-dose CHCP group (SCCl₄+1‰ CHCP,SCCl₄+2‰ CHCP and SCCl₄+4‰ CHCP respectively).CHCP were used by drinking water since 7 days.SCCl₄ were gavaged according to 2 mL/(kg·BW) at 14 and 28 d.The results showed as follows:The model of layers liver damage could be built by intragastric administration of 2 mL/(kg·BW) 10%(V/V) SCCl₄ at 14,28 d respectively,with the signs of hepatic steatosis,severe vacuolar degeneration,nuclear condensation and necrosis.Compared to the model control group,the serum AST levels in low,medium and high dose CHCP groups were decreased by 4.35% (P > 0.05),7.57% (P > 0.05) and 9.79% (P < 0.05),the serum ALT levels in medium and high dose CHCP groups were decreased by 34.92% (P < 0.01),36.51% (P < 0.01),the serum total bilirubin content in medium and high dose CHCP groups were decreased by 25.49% (P < 0.01),27.45% (P < 0.01).The liver cell congestion was reduced to varying degrees in different dose CHCP groups,and the liver cell had no vacuoles,arranged in neat rows,abundant cytoplasm and uniform in different dose CHCP groups.In conclusion,2‰,4‰ CHCP could reduce hepatocyte necrosis,decrease the serum activities of ALT,AST and total bilirubin levels,and had protective effect on hepatic injury induced by SCCl₄.  相似文献   

三七地上茎4个转录因子基因转录水平的纵向变化及其与总三萜皂苷含量的关系     

臧灵飞  张洪玲  杨迪  赵鹏宇  赵昶灵  李桂琼  古朝山  张优  顾凡  陈杞临 《贵州农业科学》2020,48(5):31-36

为三七绿紫茎三萜皂苷合成转录调控机理提供参考，将彻底长成的绿紫茎均分为18段，用实时荧光定量PCR检测茎段的三七碱性螺旋-环-螺旋蛋白基因（bHLH）、乙烯响应因子基因（ERF）、v-myb骨髓母细胞增多症病毒癌基因同源物基因（MYB）和WRKY1蛋白基因（WRKY1）的转录水平，用香草醛-高氯酸比色法检测茎段的总三萜皂苷含量（TTSC），分析转录水平与TTSC间的Pearson相关性。结果表明：从三七绿紫茎的顶部到基部，三七bHLH、ERF、MYB和WRKY1的转录水平分别呈一条波动式平缓上升、7峰、波动幅度逐渐增大的6峰和具2个主峰的6峰曲线；TTSC呈V型曲线，最低TTSC出现在茎上部的黄金分割点处；4种转录因子基因的转录水平和TTSC在不同茎段间的差异均达到极显著水平，且转录水平与TTSC之间的Pearson相关系数分别为0.371、0.130、－0.169和－0.195。因此，与三七绿紫茎三萜皂苷合成相关的主要转录因子应为三七的碱性螺旋-环-螺旋蛋白，其次为乙烯响应因子。  相似文献   

协同办公平台数据驱动下的农业科研绩效评价     

李昀  吴华瑞  李奇峰  顾静秋  李庆学  韩笑 《河北农业大学学报》2020,43(5):122-130

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利用CRISPR/Cas9技术创建OsCOL9水稻突变体     

刘维  刘浩  董双玉  古丰玮  陈志强  王加峰  王慧 《华北农学报》2017,32(4)

CRISPR/Cas9系统可对植物的内源基因进行有效定点编辑,为作物的遗传育种提供了新的方向。以感病水稻品种丽江为材料,抗病基因OsCOL9为靶基因,探索该系统对水稻内源基因定点编辑效率以及获得有研究意义的突变体。OsCOL9基因CDS序列全长1 266 bp,编码一个422 aa蛋白,分子量大小约为45.6 k Da,蛋白质结构的N端含有B-box结构域,C端含有CCT(CONSTANS、CONSTANS-Like、TOC1)结构域。设计4个长20 nt的guide RNAs(gRNAs)靶点,靶向编辑OsCOL9基因的CDS起始区域以及外显子的末端,分别由U3、U6a、U6b以及U6c启动子驱动,提取T1转基因植株的基因组DNA并对编辑位点附近的DNA片段进行序列分析。结果表明,T1材料中OsCOL9的碱基缺失数最多可达59 bp,突变次数最多可达11次,取得较好的基因编辑效果。同时本试验出现了脱靶效应,因此着重探讨了降低脱靶效应的方法。由于CRISPR/Cas9系统在进行基因编辑时没有外源基因的导入,加上该技术的快捷、简便,对生物技术与传统育种的结合具有重要实践意义。  相似文献   

读者需求驱动的“五位一体”高校图书馆文献资源建设模式研究     

顾洁  宋飞  张玉慧 《农业图书情报学刊》2019,31(7):44-49

为使图书馆文献资源建设更好的满足读者,提高读者使用资源的效率与满意度,通过文献调研法和问卷调查法对高校图书馆文献资源建设现状和读者需求情况进行调研分析,在此基础上构建出读者需求驱动的以OPAC查询、多渠道荐购、实体书店和电子资源4种渠道为主、文献传递和学科服务团队服务为辅的“五位一体”高校图书馆文献资源建设模式。总结模式的特点并辅以模式执行一段时间的数据统计。该模式五个环节应读者需求而生、为读者需求而动,互为补充,相互协调,系统地、全方位地满足了读者需求,并从该模式出发进一步探究高校图书馆文献资源建设未来发展方向。  相似文献   

野鸭椿育苗与园林应用研究   总被引：1，自引：0，他引：1  

顾建中  王燕  朱发仁 《中国园艺文摘》2014,(6):156-157

野鸭椿花色淡雅、果色红艳、观果期长,是良好的观花、观果植物,生产上可通过播种繁殖、扦插繁殖,经过4~5年可培育成大苗用于庭院造景。野鸭椿在园林中可单纯成林,也可丛植成景、孤植成主景,还可修剪成盆景,应用前景十分广阔。  相似文献   

矩形底面扁球面网壳的分岔问题     

顾小妹 《河北北方学院学报(自然科学版)》2015,(2):4-7,12

对曲面为正三角形网格矩形底面扁球面单层网壳,用拟壳法建立非线性动力学方程,在固定夹紧的边界条件下,给出满足边界条件的动态解。通过Galerkin法得到该问题的非线性动力学方程,用Floquent指数方法研究系统的分岔问题,讨论了平衡点(奇点)领域的稳定性问题。并且通过数字仿真绘出了不同平衡点处系统的分岔图,指出系统在动静载荷作用下平衡位置的变化情况。  相似文献